Spectrophotometric determination of ajmaline and brucine by Folin Ciocalteu’s reagent
نویسندگان
چکیده
A rapid and simple spectrophotometric procedure is described for the determination of ajmaline and brucine. The method is based on the development of blue coloured product due to reduction of tungstate and/or molybdate in Folin Ciocalteu’s reagent (FCR) by ajmaline and brucine in alkaline medium. The colour is stable for more than 48 h. The chromogenic reaction has max at 540 nm with molar absorptivity 1.64 10 4 and 2.37 103 l mol-1 cm-1 in the Beer’s law range 1–8 g ml-1 and 10–100 g ml-1 for ajmaline and brucine, respectively.
منابع مشابه
Simple Spectrophotometric Determination of Isoxsuprine Hydrochloride in Spiked Human Urine and in Tablets Using Folin-Ciocalteu’s Reagent
A very simple, rapid and cost effective spectrophotometric method is described for the determination of isoxsuprine hydrochloride (ISX). The method is based on the selective reduction of Folin-Ciocalteu’s reagent (F-C reagent) by phenolic and amino group present in ISX in alkaline medium to form a blue coloured chromogen which absorbs maximally at 770 nm. Beer’s law is obeyed in the concentrati...
متن کاملApplication of Differential Kinetic Method Using ANN with a New Synthetic Reagent for Simultaneous Spectrophotometric Determination of Mercury and Palladium
A new selective reagent was used for simultaneous determination of mercury and palladium in real samples with their different kinetic spectrophotometric properties. The method is based on the difference in the rate of the oxidation reaction of the recently synthesized reagent, nitro benzoyl diphenylmethylen phosphorane (N-BDMP), with Hg2+ and Pd2+. The kinetic profiles were monitored and record...
متن کاملA novel spectrophotometric determination of caroverine in pharmaceutical formulations via derivatization with Folin-ciocalteu Phenol reagent.
In this study we have reported a new, fast and extraction free spectrophotometric procedure for the assessment of caroverine in pharmaceutical raw and tablet dosage forms. In the reported visible spectrophotometric procedure tungstate in Folin-Ciocalteu phenol reagent is reduced in alkaline medium and produces the blue colored chromogen that shows Λmax at 740nm with the calibration range of 2-2...
متن کاملNovel spectrophotometric methods for the determination of phenolic β-lactam antibiotic (Cefprozil) in bulk and dosage forms
Three simple, accurate and economical spectrophotometric methods in ultraviolet and visible region were developed for the determination of cefprozil in bulk and dosage forms have been described. The method A is based on the reduction of ferric ion into ferrous ion by the mentioned drug in the presence of 1,10-phenanthroline to form a highly stable orange red colored ferrion complex measured at ...
متن کاملSynthesis of 6-(Phenanthrene-3-yl)-2,3-dihydro-1,2,4,-triazine-3-thione as a New Reagent for the Non-Extractive Spectrophotometric Determination of Cu(II)
A simple, sensitive, and non-extractive spectrophotometric method was developed for the determination of copper (II) using a newly synthesized chromogenic reagent, 6-(phenanthrene-3-yl)-1,2,4,-triazine-3-thione(PhDTT), in Tween 80 neutral surfactant. To synthesize the reagent (PhDDT), amyl nitrite was added to 3-acethylphenanthrene and refluxed for 26 hours to produce 2-oxo-2-(phenanthren-3...
متن کامل